Research Paper Volume 11, Issue 22 pp 9982—9999

Circular RNA hsa_circ_0000515 acts as a miR-326 sponge to promote cervical cancer progression through up-regulation of ELK1

Figure 2. Hsa_circ_0000515 is expressed at high levels in cervical cancer tissues and cells. (A) the expression of hsa_circ_0000515 in cervical cancer and normal adjacent tissues determined by RT-qPCR; *p < 0.05, compared with normal adjacent tissues; (B) overall survival of patients with high expression or low expression of hsa_circ_0000515 with the mean expression of hsa_circ_0000515 as the cut-off value; (C) hsa_circ_0000515 expression in normal cervical cell line (H8) and 4 cervical cancer cell lines (Hela, U14, SiHa, CaSki) determined by RT-qPCR; *p < 0.05 vs. the normal cervical cell line H8; measurement data were expressed as mean ± standard deviation; unpaired t test was used to compare data with normal distribution and equal variance between cervical cancer (n = 63) and normal adjacent tissues (n = 40); one-way ANOVA was applied for comparison of data among multiple groups, followed by Tukey's post hoc test. The survival rate of the patients was calculated by Kaplan-Meier method, and the univariate analysis was performed by Log-rank test. The cell experiments were repeated 3 times independently.