Research Paper Volume 11, Issue 22 pp 10144—10153

Long noncoding RNA LINC00899 suppresses breast cancer progression by inhibiting miR-425

Figure 4. Overexpression of LINC00899 inhibits BC cell proliferation, colony formation, migration and invasion. (A) MDA-MB-231 and SKBR3 cells were transfected with LINC00899-carrying lentivirus (Lv-LINC00899) or control lentivirus (Lv-control) followed by qRT-PCR analysis of the relative LINC00899 expression levels. (BE) Cells transfected with Lv-control, Lv-LINC00899 or Lv-LINC00899 plus miR-425 mimic. CCK8 (B) and colony formation (C) assays were used to assess proliferation of MDA-MB-231 and SKBR3 cells. Wound healing assays were performed to assess migration of MDA-MB-231 (D) and SKBR3 (E) cells. Transwell invasion assays were performed to assess invasion by MDA-MB-231and SKBR3cells (F). Data presented as means ± SD.**p< 0.01.