Tissue plasminogen activator disrupts the blood-brain barrier through increasing the inflammatory response mediated by pericytes after cerebral ischemia

Figure 2.Rt-PA disrupted the BBB in vitro. (A) Schematic of the in vitro BBB model. (B) Endothelial cells were stained with CD31, and pericytes were stained with NG2; scale bar: 50 μm. (C) We determined the TEER at 2 d, 4 d, 6 d and 8 d after construction of the in vitro BBB model; n = 5 for each group. Data represent the mean ± sd *p < 0.05, **p < 0.01. (D) Mean pericyte survival was measured after OGD for 2 h, 4 h, or 6 h; n = 6 for each group. Data represent the mean ± sd *p < 0.05, **p < 0.01. (E, F) We measured the TEER and permeability of the sham-treated mice and mice 1 d after OGD/R with or without 50 μg/ml rt-PA treatment in the in vitro BBB model; n = 3 for each group. Data represent the mean ± sd, *p < 0.05, **p < 0.01.