Research Paper Volume 11, Issue 22 pp 10183—10202

Development and validation of a metastasis-associated prognostic signature based on single-cell RNA-seq in clear cell renal cell carcinoma

Figure 3. Internal and external validation of MAGs to determine its clinical predictive value. (A, C) The AUCs of ROC curves were 0.763 and 0.803 in predicting 3-year OS events in training and testing cohorts, respectively. (B, D) Besides, Kaplan-Meier analysis indicated that patients with high MAGs-score suffered significantly worse OS outcomes (P = 2.904e-08), which was validated consistently in testing cohort with P = 1.031e-10. (E, F) In addition, we also proved our findings in an independent ICGC cohort and observed the similar statistical results. (GI) We further integrated MAGs signature with survival analysis in the total TCGA-KIRC cohort and distribution plots suggested that high MAGs risk scores correlated with more dead and recurrence/progression cases.