Research Paper Volume 11, Issue 23 pp 10814—10825

Long noncoding RNA LINC00958 accelerates the proliferation and matrix degradation of the nucleus pulposus by regulating miR-203/SMAD3

Figure 4. Ectopic expression of LINC00958 promoted NP cell proliferation. (A) Ectopic expression of LINC00958 promoted NP cell growth, which was determined by using the MTT assay. (B) Overexpression of LINC00958 increased cyclin D1 expression in the NP cell. (C) Elevated LINC00958 expression enhanced PCNA expression in the NP cell. (D) LINC00958 overexpression promoted the protein expression of cyclin D1 and PCNA. *p<0.05, **p<0.01 and ***p<0.001. GAPDH was used as the loading control. Data were showed as mean±SD.