Research Paper Volume 11, Issue 23 pp 10814—10825

Long noncoding RNA LINC00958 accelerates the proliferation and matrix degradation of the nucleus pulposus by regulating miR-203/SMAD3

Figure 7. LncRNA LINC00958 exerted its function by targeting miR-203 in the NP cells. (A) Cell proliferation was measured by using the MTT assay. (B) PCNA expression was determined by qRT-PCR. (C) The expression of cyclin D1 was measured by qRT-PCR. (D) Elevated expression of LINC00958 suppressed the expression of MMP-3 in the LINC00958-overexpressing NP cells. (E) The expression of MMP-13 was determined by qRT-PCR analysis. (F) Col II expression was measured by using qRT-PCR analysis. (G) The expression of aggrecan was detected by using qRT-PCR analysis. *p<0.05, **p<0.01. GAPDH was used as the internal control. Data were showed as mean±SD.