Research Paper Volume 11, Issue 22 pp 10220—10241

Circular RNA CircCCNB1 sponges micro RNA-449a to inhibit cellular senescence by targeting CCNE2

Figure 2. Confirmation of SAC-RNAs expression profiles in prematurely senescent and RS 2BS fibroblasts. (A) Senescence-associated beta-galactosidase (SA-β-gal) staining of in mock-treated (proliferating) and irradiation-induced (prematurely senescent) 2BS cells, bars, 100 μL. (B) Immunoblots analysis of P53 and P21levels in mock-treated and irradiation-induced premature senescent 2BS. (C) Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis of alterations of CircRNA expression in mock-treated and irradiation-induced 2BS cells. (D) SA-β-gal staining of proliferating and RS 2BS cells, bars, 100 μL. (E) immunoblots analysis of the levels of P53 and P21 in proliferating and RS 2BS cells. (F) qRT-PCR analysis of changes in CircRNAs expression in proliferating and RS 2BS cells.