Figure 6. CCNE2 is directly regulated by miR-449a and indirectly regulated by CircCCNB1. (A) Schematic illustration of CCNE2 3′-UTR wild-type (wt) and CCNE2 3′-UTR-mutant (mut) dual luciferase reporter vectors. (B) The relative luciferase activities were detected in 293T cells after transfection with CCNE2 3′-UTR wt and CCNE2 3′-UTR -mut and miR-449a mimics or miR-NC, respectively, *P<0.05. (C and D) Relative mRNA and protein levels of CCNE2 in 2BS or IMR-90 cells after transfection with miR-NC, miR-449a, inhibitor-NC and inhibitor-449a using qRT-PCR and western blot, respectively, **P<0.01.