Research Paper Volume 11, Issue 22 pp 10220—10241

Circular RNA CircCCNB1 sponges micro RNA-449a to inhibit cellular senescence by targeting CCNE2

Figure 7. CircCCNB1 suppresses cellular senescence by sponging miR-449a to regulate CCNE2. (A and B) CCK-8 analysis was performed in 2BS or IMR-90 cells after transfection with ctrl, CircCCNB1 and CircCCNB1+miR-449a mimics, or sh-scramble, sh-CircCCNB1-1 and sh-CircCCNB1-1+miR-449a inhibitors, respectively, **P<0.01. (C and D) Clonogenicity assay of 2BS or IMR-90 cells after transfection with ctr, CircCCNB1 and CircCCNB1+miR-449a mimics, or sh-scramble, sh-CircCCNB1-1 and sh-CircCCNB1-1+miR-449a inhibitors, respectively, **P<0.01, ***P<0.001. (E and F) SA-β gal of 2BS or IMR-90 after transfection with sh-scramble, sh-CircCCNB1-1 and sh-CircCCNB1-1+miR-449a inhibitors respectively, bar, 100μm, **P<0.01, ***P<0.001. (G and H) Immunoblot analysis of CCNE2, P53 and P21 levels in 2BS or IMR-90 cells after transfection with ctr, CircCCNB1 and CircCCNB1+miR-449a mimics, or sh-scramble, sh-CircCCNB1-1 and sh-CircCCNB1-1+miR-449a inhibitors, respectively.