Figure 7. Research protocol. (A) An overview of the experimental scheme. (B) Graphic illustrating the platelet preparation from the tube-method protocol. The extraction of a small volume of whole blood (WB) was obtained from SAM-mice. After the single centrifugation at 150g for 20min, blood was fractionated into two layers: the bottom layer consists of red blood cells (RBC), and the top layer contains platelet rich plasma (PRP). The separated PRP was then centrifuged at 400g for 15min: the bottom consists of platelet pellets (PLT), and the top contains platelet poor plasma (PPP).