Research Paper Volume 11, Issue 22 pp 10252—10265

Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release

Figure 1. Stem cell marker expression of rabbit AF cells tested by immunostaining. (AC) nucleostemin testing; (DF) Oct-4 testing; (GI) SSEA-4 testing. (A, D, G) the cells were stained with H33342; (B, E, L) the cells were stained with specific antibodies; (C, F, I) the merged images of the images of A, D, G and the images of B, E, L. The insets showed enlarged views of expressed nucleostemin (C) and Oct-4 (F). (J) Semi-quantification of the expression of three stem markers by immunostaining. The results indicated that more than 92% of the cells isolated from rabbit AF tissues were stem cells. Bars = 100 μm.