Research Paper Volume 11, Issue 22 pp 10252—10265

Metformin decreases LPS-induced inflammatory response in rabbit annulus fibrosus stem/progenitor cells by blocking HMGB1 release

Figure 2. Metformin effect on the proliferation of rabbit AF cells cultured for 7 days. Metformin didn’t induce the morphological change significantly in rabbit AF cells during the culture (AD), while the proliferation of the rabbit AF cells was decreased at a metformin concentration dependent manner as evidenced by population doubling time (E). *p<0.05 compared to the control group (Met-0, Met-1, Met-5, and Met-10 represent that the concentrations of metformin in the medium are 0, 1, 5, 10 mM, respectively). White bars = 100 μm.