Research Paper Volume 11, Issue 22 pp 10266—10283

Silencing lncRNA FOXD2-AS1 inhibits proliferation, migration, invasion and drug resistance of drug-resistant glioma cells and promotes their apoptosis via microRNA-98-5p/CPEB4 axis

Figure 4. Silencing of FOXD2-AS1 contributes to inhibition of proliferation and drug resistance of drug-resistant glioma cells and promotion of their apoptosis. (A) The expression differences of FOXD2-AS1 in drug-resistant cell lines (U87TR, U251TR) and parental cell lines (U87, U251) were detected by RT-qPCR. (B) EdU assay was used to detect cell proliferation activity. (C) Changes of IC50 in drug-resistant cell lines after silencing FOXD2-AS1; (D) Cell apoptosis was detected by flow cytometry; (E) Tumor growth in nude mice was detected by tumor formation experiment; * P < 0.05 vs the sh-NC group; The data were all measurement data, represented by mean ± standard deviation. The comparison between the two groups was statistically analyzed by independent sample t test, and the experiment was repeated for three times.