Research Paper Volume 11, Issue 22 pp 10266—10283

Silencing lncRNA FOXD2-AS1 inhibits proliferation, migration, invasion and drug resistance of drug-resistant glioma cells and promotes their apoptosis via microRNA-98-5p/CPEB4 axis

Figure 5. Overexpression of FOXD2-AS1 accelerates the proliferation and drug resistance of drug-resistant cells in glioma and inhibits their apoptosis. (A) The expression of FOXD2-AS1 in U87TR cell line was detected by RT-qPCR; (B) The expression of FOXD2-AS1 in U251TR cell line was examined by RT-qPCR; (C) The cell proliferation activity tested via EdU assay; (D) The changes of IC50 in drug-resistant cell lines after overexpression of FOXD2-AS1; (E) The apoptosis in U87TR and U251TR cell lines tested via flow cytometry; (F) The tumor growth tested via tumor xenograft in nude mice. * P < 0.05 vs the oe-NC group; The data in the figure were all measurement data, represented by mean ± standard deviation. The comparison between the two groups was statistically analyzed by independent sample t test, and the experiment was repeated for three times.