Research Paper Volume 11, Issue 22 pp 10266—10283

Silencing lncRNA FOXD2-AS1 inhibits proliferation, migration, invasion and drug resistance of drug-resistant glioma cells and promotes their apoptosis via microRNA-98-5p/CPEB4 axis

Figure 6. LncRNA FOXD2-AS1 is regarded as a sponge of miR-98-5p. (A) The subcellular localization of FOXD2-AS1 was verified by FISH assay. (B) The binding site of FOXD2-AS1 and miR-98-5p were predicted in the RNA22 website. (C) The binding of FOXD2-AS1 to miR-98-5p was verified by dual luciferase reporter gene assay, miR-98-5p-MUT was MUT plasmid of FOXD2-AS1 and miR-98-5p 3′UTR, and miR-98-5p-WT was WT plasmid of FOXD2-AS1 and miR-98-5p 3′UTR; (D) The binding of FOXD2-AS1 to Ago2 was detected by RIP assay. (E) RNA pull-down assay was used to detect the enrichment of FOXD2-AS1 by miR-98-5p. (F) Expression of miR-98-5p in glioma tumor tissues was detected by RT-qPCR, N = 86; * P < 0.05 vs the oe-NC group, IgG group, Bio-probe NC group or para normal tissues; The data were all measurement data, represented by mean ± standard deviations. The comparison between the two groups was statistically analyzed by independent sample t test, and one-way ANOVA was used in comparisons among multiple groups, and Tukey’s post-hoc test was performed after ANOVA. The experiment was repeated for three times.