Research Paper Volume 11, Issue 22 pp 10266—10283

Silencing lncRNA FOXD2-AS1 inhibits proliferation, migration, invasion and drug resistance of drug-resistant glioma cells and promotes their apoptosis via microRNA-98-5p/CPEB4 axis

Figure 7. FOXD2-AS1 acts as a ceRNA to adsorb miR-98-5p, thereby decreasing the expression of CPEB4. (A) The target relationship between miR-98-5p and CPEB4 was predicted by Targetscan website. (B) The targeting relationship between miR-98-5p and CPEB4 was verified through dual luciferase reporter gene assay, CPEB4-MUT was MUT plasmid of miR-98-5p and CPEB4 3′UTR, and CPEB4-WT was WT plasmid of miR-98-5p and CPEB4 3′UTR; (C) CPEB4 expression in glioma tumor tissues was detected by RT-qPCR, N = 86; (D) The expression levels of FOXD2-AS1, miR-98-5p and CPEB4 in cells after transfection were detected by RT-qPCR. * P < 0.05 vs the mimic-NC group, para normal tissues, the oe-FOXD2-AS1 + mimic NC group, the sh-FOXD2-AS1 + inhibitor NC group or the miR-98-5p inhibitor + sh-NC group; The data were all measurement data, represented by mean ± standard deviation. The comparison between the two groups was statistically analyzed by independent sample t test, and the experiment was repeated for three times.