Research Paper Volume 11, Issue 22 pp 10284—10300

The anti-carcinogenesis properties of erianin in the modulation of oxidative stress-mediated apoptosis and immune response in liver cancer

Figure 5. Erianin inhibited HepG2-xenograft tumor growth in BALB/c mice. After three consecutive injections of cyclophosphamide, BALB/c mice inoculated with HepG2 cells were treated with erianin (20 mg/kg dissolved in 0.9% saline solution containing 1:10,000 DMSO) or vehicle solvent (0.9% saline solution containing 1:10,000 DMSO) for 24 days. (A) Tumor-bearing mice and (B) tumors collected from vehicle- and erianin-treated groups. (C) Mean (±SD) body weights in the erianin-treated and vehicle groups (n = 6). (D) Pathological analysis of liver and spleen tissues via H&E staining. (E) Effects of erianin on 111 types of cytokines in mice tumors detected by Mouse XL Cytokine Kit. The arrows indicate the factors for further detection. 1. CCL2; 2. CCL11; 3. CCL21; 4. CXCL11; 5. CXCL13; 6. CXCL16; 7. GM-CSF; 8. IL-6; 9. IL-10; 10. MMP-2; 11. MMP-9; 12. TNF-α. (F) Scatter diagram of 111 cytokines. The relative density is the ratio of the absolute value and the reference spot value. The red dots indicate the factors with a change of >50% compared with control mice.