Research Paper Volume 11, Issue 22 pp 10284—10300

The anti-carcinogenesis properties of erianin in the modulation of oxidative stress-mediated apoptosis and immune response in liver cancer

Figure 8. Effects of erianin on oxidative stress-mediated NF-κB pathway. (A) Erianin enhanced the levels of Nrf2, HO-1, SOD-1, and SOD-2, and reduced the phosphorylation levels of Erk1/2, IKKα/β, and NF-κB in spleens of BALB/c mice bearing HepG2- and SMMC-7721-xenografted tumors. Quantitative protein expression was normalized to GAPDH levels and/or related total protein levels in the corresponding samples. The marked average changes of proteins were expressed as folds relative to the corresponding control tumor tissues (n = 6). (B) Erianin enhanced the RNA levels of HO-1 and SOD-1 in spleens of BALB/c mice bearing HepG2- and SMMC-7721-xenografted tumors. Quantitative RNA expression data were normalized to the corresponding β-actin levels. The marked average changes of HO-1 and SOD-1 were expressed as folds relative to the corresponding control tumor tissues (n = 6). The molecular mass from top to bottom of the marker is: 1000, 700, 500, 400, 300, 200, 100 (bp). (C) Erianin reduced the phosphorylation levels of NF-κB in cytoplasm, and inhibited its transfer from cytoplasm to nucleus. The quantitative P-NF-κB expression in the cytoplasm and nucleus was normalized to the T- NF-κB levels, respectively. The marked average changes were expressed as folds relative to the corresponding control tumor tissues (n = 6).