Research Paper Volume 11, Issue 22 pp 10356—10373

Folic acid delays age-related cognitive decline in senescence-accelerated mouse prone 8: alleviating telomere attrition as a potential mechanism

Figure 3. FA supplementation delayed neurodegeneration and stimulated neuronal survival in cerebral cortex and hippocampal CA1 region in SAMP8 mice. Mice were assigned to treatment groups as described in Figure 1. Representative micrographs of HE staining in cerebral cortex (A) and hippocampal CA1 region (B), Nissl staining in cerebral cortex (C) and hippocampal CA1 region (D), Fluoro-Jade B (FJB) staining in cerebral cortex (E) and hippocampal CA1 region (F). Quantitative analysis of surviving neurons indicated by Nissl staining in cerebral cortex [F(5,24) = 30.882, P<0.001] (G) and hippocampal CA1 region [F(5,24) = 68.256, P<0.001] (H), and degenerated neurons indicated by FJB-positive cells in cerebral cortex [F(5,24) = 44.797, P<0.001] (I) and hippocampal CA1 region [F(5,24) = 45.630, P<0.001] (J). Yellow arrow indicates FJB positive cell. Scale bar = 50 μm. Data are expressed as mean ± SD (n= 5 mice/group). *P<0.05 compared with FA-N group. #P<0.05 compared with Con-Y group.