Research Paper Volume 11, Issue 22 pp 10374—10384

Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Figure 1. Hypoxia induced miR-10b-3p expression and its target validation. ECA109 cells transfected with sh-HIF-1α or pcDNA- HIF-1α were incubated for 48 hours under normoxic or hypoxic conditions. (A) Western blotting showed HIF-1α expression. (B) Quantification of miR-10b-3p expression using qRT-PCR. (C) ECA109 and KYSE410 cells were transfected with miR-10b-3p mimics or mimics NC, after which TSGA10 expression was assessed by western blotting. (D) Predicted miR-10b-3p wild-type binding sites (WT) or mutant binding sites (MUT) were cloned into a luciferase reporter. Cells co-transfected with miR-10b-3p mimics or controls and WT or MUT luciferase constructs were subjected to luciferase assay. (E) Measurement of luciferase activity. *P < 0.05.