Figure 4. MiR-10b-3p contributes to ESCC tumor growth and metastasis in vivo. (A) An ESCC xenograft model in BALB/c nude mice was constructed using ECA109 cells. MiR-10b-3p agomir or agomir NC was injected into the tumor mass as indicated. After 40 days, tumors that developed were excised and photographed. (B) Tumor volumes were measured every 5 days and plotted. (C and D) To assess cell proliferation, tumor sections were immunostained for Ki-67 and photographed (C), after which Ki-67-positive cells were quantified (D). (E) Representative images of hematoxylin- and eosin-stained metastases in lungs. (F) Quantification of metastatic lung nodules. *P < 0.05.