Figure 4. Effect of lnc RNF7 silence on CF rat (A) CF rats were injected with Lv-sh-lnc RNF7 or Lv-sh-NC and examined for the infection efficiency by real-time PCR. (B) Pathomorphological features of rat hearts in different groups examined by Masson staining. (C) The fibrotic areas were calculated and the percentage of fibrotic tissue area was used to assess CF. (D–F) The mRNA expression and protein levels of Col1a1 (Collagen I), Tgfb1 (TGFβ1), Ctgf (CTGF), FN1 (Fibronection), and ACTA1 (α-SMA) in CF and control groups determined by real-time PCR and Immunoblotting analyses.