Figure 3. Overexpression of circ5912 suppresses bladder cancer growth and metastasis. Bladder cancer cell lines with overexpressed circ5912 were designed and produced. (A) qPCR detected levels of circ5912 and FIP1L1 after circ5912 overexpression; (B, C) a CCK8 assay was performed to evaluate cell viability; (D, E) clone-forming ability was detected; (F, G) wound healing ability was measured by the distance between the two sides of induced injury after 24 hours, scale bar: 100μm; (H–K) migration and invasion were assessed by counting cells that penetrated the trans-well membrane, scale bar: 25μm; (L–N) the in vivo effect of circ5912 was evaluated by subcutaneously injecting circ5912 overexpressing cells into nude mice. Mice were killed 4 weeks after injection, and tumor weight and volume were measured.