Figure 11. The molecular mechanism underlying the anti-aging effect of RMF. (A) The mRNA levels of hsp-16.1, hsp-16.2, age-1, sir-2.1, ctl-1, skn-1, gst-4 and gcs-1 were determined by quantitative real-time RT-PCR and normalized to the expression of act-1. At least 3000 C. elegans were used in each group and the experiments were performed on three independent occasions. Means with different letters were significantly different at P < 0.05 for each gene. (B) RMF extended lifespan of the hsf-1 (AGD794) and eat-2 (DA1116) mutants. RMF did not extend lifespan of the daf-2 (CB1370) and daf-16 (CR1307) deletion mutants. The lifespan analysis of each mutant was performed on three independent occasions. (C) A possible mode of action of the longevity extension mediated by RMF.