Research Paper Volume 11, Issue 22 pp 10385—10408

Rotating magnetic field delays human umbilical vein endothelial cell aging and prolongs the lifespan of Caenorhabditis elegans

Figure 11. The molecular mechanism underlying the anti-aging effect of RMF. (A) The mRNA levels of hsp-16.1, hsp-16.2, age-1, sir-2.1, ctl-1, skn-1, gst-4 and gcs-1 were determined by quantitative real-time RT-PCR and normalized to the expression of act-1. At least 3000 C. elegans were used in each group and the experiments were performed on three independent occasions. Means with different letters were significantly different at P < 0.05 for each gene. (B) RMF extended lifespan of the hsf-1 (AGD794) and eat-2 (DA1116) mutants. RMF did not extend lifespan of the daf-2 (CB1370) and daf-16 (CR1307) deletion mutants. The lifespan analysis of each mutant was performed on three independent occasions. (C) A possible mode of action of the longevity extension mediated by RMF.