Figure 2. Rotating magnetic field exposure delays HUVEC senescence. (A, B) HUVECs were exposed to RMF daily for 0 h, 2 h, and 4 h; 20 μM metformin was used as a positive control. SA-β-Gal staining was performed and the number of β-Gal-positive (blue) cells was calculated as a percentage of the total cell number using Image J software. (C, D) Flow cytometry was used to detect the apoptosis of HUVEC after RMF treatment. (E) Western blot analysis showed that RMF exposure increased in AMPK protein expression and decreased P21, P53 and mTOR protein expression.