Research Paper Volume 11, Issue 22 pp 10485—10498

Identification of functional tRNA-derived fragments in senescence-accelerated mouse prone 8 brain

Figure 2. Validation of tRFs expression by quantitative polymerase chain reaction (qPCR). The U6 gene was used as a housekeeping internal control. The relative expression of each tRF was represented as mean ± SEM [n = 3, three mice per group (biological replicates), three times per mouse (technical replicates)]. *p < 0.05, **p < 0.01, ***p < 0.001, ns means nonsignificant.