Research Paper Volume 11, Issue 22 pp 10499—10512

Melatonin modulates IL-1β-induced extracellular matrix remodeling in human nucleus pulposus cells and attenuates rat intervertebral disc degeneration and inflammation

Figure 3. Effects of 200 μM melatonin and 5 nM IL-1β for 24 h on collagen II (COL2), aggrecan and MMP-3 in NPCs. (A) Protein levels of COL2, aggrecan, MMP-3. (B) Semi-quantitative analysis of COL2 levels (n=3). (C) Semi-quantitative analysis of aggrecan levels (n=3). (D) Semi-quantitative analysis of MMP-3 levels (n=3). The values are expressed as mean ± SD. **P<0.01 vs control group; ##P<0.01 vs IL-1β group.