Figure 2. Analysis of proliferation state of cells in culture. Representative double staining for Click-iT EdU (green) and FOXL2 (red) on cultured cells (A–C) and isolated secondary follicles (D) after 24 hrs of culture. Orange and white arrowheads indicate proliferating FOXL2 positive and negative cells, respectively (A’-A’’’ higher magnification images from A). © GCs in large colonies and (D) GCs spreading out from secondary follicles were negative for Click-iT EdU proliferation assay. Scale bar = 100μm.