Figure 8. LH modulates the H2AX phosphorylation in somatic ovarian cells. (A) Representative IF for γH2AX in cultured ovarian somatic cells treated with 10 μM CS with/out 200 mIU/mL LH for the indicated times. Scale bar = 100 μm. (B) The graph reports the quantification of the percentage of cells positive for γH2AX. Data are shown as mean ± SEM of five experiments. Statistical differences vs control *p<0.05 ****p<0.0001; Cs+LH group vs CS group b = p<0.01 d = p<0.0001. (C, D) Representative WB of γH2AX in cells treated with CS with/out LH for the indicated times. Note a clear reduction of γH2AX level at 72 hrs. Data are expressed as mean ± SEM of three experiments. Statistical differences vs control **p<0.01 ***p<0.001 ****p<0.0001; statistical difference of CS group vs CS+LH group b = p<0.01 c = p<0.001.