Figure 2. SRF overexpression induced EMT and migration in NRK-52E cells. (A) Western blot analysis showing the induction of SRF, α-SMA, and FSP-1 expression and a reduction in E-cadherin expression. (B) Quantitative RT-PCR analysis of SRF mRNA expression. (C) Representative micrographs of the transwell chamber migration assay at an original magnification of 200×. Arrowheads indicate some of the migrated cells. Bar=50 μm. (D) Quantitative analysis of the number of migrated NRK-52E cells per field in three groups. *P<0.05 versus the control group; #P<0.05 versus the pcDNA3.1 group. All experiments were repeated three times.