Research Paper Volume 11, Issue 22 pp 10597—10609

Crucial role of serum response factor in renal tubular epithelial cell epithelial-mesenchymal transition in hyperuricemic nephropathy

Figure 3. Inhibition of SRF preserved the phenotype of NRK-52E cells after UA stimulation in vitro. NRK-52E cells were pretreated with CCG-1423 (1 μM or 2 μM) or DMSO for 1 h, followed by treatment with UA (15 mg/dL) for 72 h. (A) Protein expression of pSRF, SRF, E-cadherin, α-SMA and FSP-1 measured by western blot analysis. (B) Quantitative determination of the pSRF and SRF protein levels. (C) Immunofluorescence staining for SRF at an original magnification of 400×. CCG-1423 was used at a concentration of 2 μM. *P<0.05 versus the control group; #P<0.05 versus the UA group. All experiments were repeated three times.