Research Paper Volume 11, Issue 22 pp 10597—10609

Crucial role of serum response factor in renal tubular epithelial cell epithelial-mesenchymal transition in hyperuricemic nephropathy

Figure 5. Inhibition of SRF improved the epithelial phenotype of TECs, renal tubulointerstitial fibrosis and albuminuria in vivo. (AC) Protein expression levels of pSRF, SRF, E-cadherin, α-SMA, FSP-1 and slug measured by western blot and quantitative analyses. (D) Renal tubulointerstitial fibrosis in the three groups measured by PAS staining. Immunohistochemical staining for slug, E-cadherin, FN and FSP-1 in the three groups. (E) Tubulointerstitial fibrosis scores of the three groups. (F) Dose-dependent reduction in 24-h UAE in HN rats induced by CCG-1423 treatment. Bar=25 μm. N=6 in each group. *P<0.05 versus the control group; #P<0.05 versus the OA group.