Figure 5. Circ_0006332 promotes bladder cancer cell proliferation, colony formation, and invasiveness by binding and inhibiting miR-143. (A) Predicted wild-type (Wt) and mutant (Mut) miR-143 binding sites in the sequence of circ_0006332 is shown. (B) Representative images of the FISH analysis show co-localization of circ_0006332 with miR-143 in the cytoplasm of bladder cancer cells. Scale bar: 50 μm. (C) Colony formation assay results show that bladder cancer cells transfected with miR-143 mimics form smaller and fewer colonies compared with the controls. (D) EdU detection assays shows that bladder cancer cells transfected with miR-143 mimics decreases the proportion of cells undergoing DNA synthesis compared with the controls. (E) Representative images of the Transwell assay show decreased invasiveness of the bladder cancer cells transfected with miR-143 mimics compared with the controls. Scale bar: 50 μm. Note: All experiments were repeated thrice; data is represented as mean ± SD; NS, no significance; *P < 0.05, **P < 0.01, ***P < 0.001.