Research Paper Volume 11, Issue 23 pp 10839—10860

HIF-2α upregulation mediated by hypoxia promotes NAFLD-HCC progression by activating lipid synthesis via the PI3K-AKT-mTOR pathway

Figure 3. Hypoxia-mediated upregulation of HIF-2α expression activates mTOR signalling pathways. (A) Pearson correlation analysis of the correlation between HIF-2α expression and relative genes in the patients with liver hepatocellular carcinoma (LIHC) from the TCGA database (http://gepia.cancer-pku.cn). (B) Western blot analysis of HIF-2α expression and expression of PI3K-AKT-mTOR pathway factors in HCC and steatotic HCC under hypoxic conditions. β-Actin was used as the loading control. Densitometric analyses of the band intensity ratios for HIF-2α/β-actin, PI3K/β-actin, p-GSK3β/GSK3β, p-AKT/AKT and p-mTOR/mTOR.