Research Paper Volume 11, Issue 23 pp 10839—10860

HIF-2α upregulation mediated by hypoxia promotes NAFLD-HCC progression by activating lipid synthesis via the PI3K-AKT-mTOR pathway

Figure 5. HIF-2α induces steatotic HCC lipid synthesis under a hypoxic microenvironment (A) Oil red O staining and quantification in HCC and steatotic HCC under hypoxic conditions in the presence or absence of MHY1485. (B, C) Triglyceride levels in HCC and steatotic HCC under hypoxic conditions in the presence or absence of MHY1485. (D) Western blot analysis of lipid synthesis-related proteins in HCC and steatotic HCC under hypoxic conditions. Densitometric analyses of the band intensity ratios for ACC1/β-actin and FASN/β-actin.