Figure 7. Hypoxia-induced HIF-2α overexpression promotes STAM mouse tumour development via activating the PI3K-AKT-mTOR signalling axis. (A) Western blot analysis of HIF-2α expression and expression of PI3K-mTOR pathway factors in STAM mice of different groups. β-Actin was used as the loading control. Densitometric analyses of the band intensity ratios for HIF-2α/β-actin, PI3K/β-actin and mTOR/β-actin. (B, C) The liver sections were stained with DAPI to visualise nuclei (blue), Alexa Fluor 488 to visualise the distribution of HIF-2α (green) and Alexa Fluor 568 to PI3K/mTOR (red) proteins. Scale bar: 10 μm.