Figure 3. Grik1 mediates KA-induced ER stress. (A, B) N2a cells were treated with 10 μM KA in the absence or presence of 2 μM topiramate for 48 h. (C, D) Grik1 siRNA (200 ng) was transfected to N2a cells before treatment with KA (10 mg/kg). The protein levels of ATF6, GRP78, and IRE1 were determined by western blots. The optical density of bands in western blots was analyzed by Image J software (*P < 0.05 vs. the control group; #P < 0.05; ##P < 0.01 vs. the KA-only treatment).