Figure 2. Mangiferin promotes macrophage cholesterol efflux and enhances RCT in apoE-/- mice. (A) [3H]-cholesterol-labeled RAW264.7 cells were intraperitoneally injected into apoE-/- mice. The amounts of [3H]-tracer in the liver, feces and plasma were assessed by LSC. *P < 0.05 vs. control group. (B–D) RAW264.7 macrophage-derived foam cells were treated with mangiferin at different concentrations (0, 5, 10, and 20 μM) for 24 h. Then, the percent cholesterol efflux to apoA-1 (B) or HDL (C) was analyzed by LSC. Lipid droplet content was assessed using Oil Red O staining (D). All results are presented as the mean ± SEM from three independent experiments, each performed in triplicate. *P < 0.05 vs. 0 μM group.