Research Paper Volume 11, Issue 23 pp 10992—11009

Mangiferin promotes macrophage cholesterol efflux and protects against atherosclerosis by augmenting the expression of ABCA1 and ABCG1

Figure 3. Mangiferin promotes the expression of ABCA1 and ABCG1 in RAW264.7 macrophages and in the aortas of apoE-/- mice. (AD) RAW264.7 macrophage-derived foam cells were exposed to different concentrations of mangiferin (0, 5, 10, and 20 μM) for 24 h. Then, the protein (A, B) and mRNA (C, D) levels of ABCA1 and ABCG1 were determined by western blot and RT-qPCR analyses, respectively. *P < 0.05 vs. 0 μM group. (EF) The mice were divided and treated as described above. The protein levels of ABCA1 and ABCG1 in the homogenate of the aortic arch were detected by western blotting. *P < 0.05 vs. control group. Data are presented as the mean ± SEM (n =3/group).