Research Paper Volume 11, Issue 23 pp 10992—11009

Mangiferin promotes macrophage cholesterol efflux and protects against atherosclerosis by augmenting the expression of ABCA1 and ABCG1

Figure 4. Mangiferin enhances the expression of PPARγ and LXRα in RAW264.7 macrophages and in the aortas of apoE-/- mice. (AF) After RAW264.7 cells were fully differentiated, the cells were exposed to different concentrations of mangiferin (0, 5, 10, and 20 μM) for 24 h. Then, RT-qPCR and western blot analyses were performed to detect the mRNA (A, D) and protein (B, C, E, F) levels, respectively, of PPARγ and LXRα. *P < 0.05 vs. 0 μM group. (G, H) Protein levels of PPARγ and LXRα in the homogenate of the aortic arch were assessed by western blotting. *P < 0.05 vs. control group. All results were collected from three independent experiments, each performed in triplicate. Data are presented as the mean ± SEM (n =3/group).