Research Paper Volume 11, Issue 23 pp 10992—11009

Mangiferin promotes macrophage cholesterol efflux and protects against atherosclerosis by augmenting the expression of ABCA1 and ABCG1


Figure 5. Mangiferin promotes ABCA1 and ABCG1 expression by upregulating LXRα levels. RAW264.7 macrophage-derived foam cells were pretreated with 2 μM T0901317 (AC) or transfected with 20 nM LXRα siRNA (DF) and then incubated with mangiferin (20 μM) for 24 h. RT-PCR and western blot analyses were performed to assess the mRNA and protein levels, respectively, of ABCA1/G1 and LXRα. All results were obtained from three independent experiments, each performed in triplicate. Data are expressed as the mean ± SEM (n =3/group). *P < 0.05 vs. control group; #P < 0.05 vs. mangiferin only group.