Figure 6. PPARγ mediates the positive effects of mangiferin on the expression of ABCA1/G1 and LXRα. RAW264.7 macrophage-derived foam cells were pretreated with 25 μM rosiglitazone (A–C) or transfected with 20 nM PPARγ siRNA (D–F) and then incubated with mangiferin (20 μM) for 24 h. RT-PCR and western blot analyses were performed to assess the mRNA and protein levels, respectively, of ABCA1/G1, LXRα and PPARγ. All results were obtained from three independent experiments, each performed in triplicate. Data are expressed as the mean ± SEM (n =3/group). *P < 0.05 vs. control group; #P < 0.05 vs. mangiferin only group.