Research Paper Volume 11, Issue 23 pp 11040—11053

Sphingosine-1-phosphate promotes PDGF-dependent endothelial progenitor cell angiogenesis in human chondrosarcoma cells

Figure 2. Overexpression of SphK1 facilitates in PDGF-A expression and angiogenesis in human chondrosarcoma. (A, B) Chondrosarcoma cells were transfected with SphK1 cDNA; SphK1 and PDGF-A expression was examined by qPCR and Western blot assays (n=5). (C, D) The CM was applied to EPCs and analyses assessed migratory and tube formation activity (n=4). Results are expressed as the mean ± SEM. *p < 0.05 as compared with the vector group.