Figure 3. Generation, isolation, and characterization of EGFR-specific CAR T lymphocytes. (A) Schematic illustration of Con-CAR, EGFR-CAR-1, and EGFR-CAR-2. (B) Expression of exogenous CD3ζin non-transduced T cells, con-CAR T cells, EGFR-CAR-1 T cells, and EGFR-CAR-2 T cells was measured using Western blots; β-actin was used as an endogenous control. (C) GFP and EGFR-GFP antigens were detected by Western blot. (D) Transduced T cells were stained with GFP and EGFR-GFP antigen and then detected by flow cytometry.