Figure 1. α-Man ameliorates inflammatory responses in eWAT from LPS-treated mice. (A) The serum levels of IL-6, TNF-α and MCP-1 were determined by ELISA kits. (B) The levels of IL-6, TNF-α and MCP-1 in eWAT were determined by ELISA kits. (C) qRT-PCR analyses for pro-inflammatory cytokines expression in eWAT. (D) qRT-PCR analyses for chemokines in eWAT, including Mcp-1, Mip-1α, Cxcl10, Ccl11, Cx3cl1 and Ccl5. (E) qRT-PCR analyses for macrophage markers in eWAT, including F4/80, Cd68, Cd11c, Cd206 and Arg-1. Data are expressed as means ± SD (n = 5). ##P < 0.01, LPS vs. control, *P < 0.05, **P < 0.01, α-Man + LPS vs. LPS.