Figure 4. α-Man ameliorates adiposity, insulin resistance, and hyperlipidemia in old mice. (A) The procedure of α-Man treatment in old mice. (B) Effect of α-Man on body weight in old mice. (C) The raw tissue weights and organ indexes for each group of mice. Fasting blood glucose (D), fasting insulin levels (E), and HOMA-IR (F) for each group of mice. (G) The levels of p-AKT and AKT in eWAT were detected by Western blots. β-Actin was used as internal loading control. (H) The levels of serum TC, TG, LDL-C, and HDL-C in serum was detected. Data are expressed as means ± SD (n = 5). #P < 0.05, ##P < 0.01, old mice vs. young mice. *P < 0.05, **P < 0.01, α-Man vs. old mice. Y, young mice; O, old mice; L, old mice administrated with 25 mg/kg α-Man; H, old mice administrated with 50 mg/kg α-Man.