Figure 5. α-Man mitigates age-related adipose tissue inflammation. (A) H&E staining of eWAT (black scale bar = 50 μm, blue scale bar = 100 μm). (B) Whole-mount immunohistochemistry analysis of the nuclei (blue), perilipin (green), and F4/80 (red), scale bar = 75 μm. The CLSs are indicated by arrows (C) ATM subtypes were quantified as a percentage of the total ATMs population using flow cytometry. (D) qRT-PCR analyses for macrophage markers in eWAT, including F4/80, Cd68, Cd11c, and Cd206. (E) qRT-PCR analyses for chemokines in eWAT, including MCP-1, MIP-1α, Cx3cl1, and Ccl5. Data are normalized to the mean value of old group. Data are expressed as means ± SD (n = 5). #P < 0.05, ##P < 0.01, old mice vs. young mice, *P < 0.05, **P < 0.01, α-Man vs. old mice. Y, young mice; O, old mice; L, old mice administrated with 25 mg/kg α-Man; H, old mice administrated with 50 mg/kg α-Man.