Figure 3. Ninj2 shRNA or KO inhibits human glioma cell proliferation. A172 glioma cells (A–C, E–G), U251MG glioma cells (D) or the primary human glioma cells (derived two patients, “P1/P2”, D) were transduced with lentiviral Ninj2 shRNAs (“sh-Ninj2”, two different sequences “Seq1/Seq2”), control shRNA (“sh-C”) or the CRISPR/Cas9 Ninj2 KO construct (“ko-Ninj2”), stable cells were established via puromycin selection; Cell proliferation was examined by the assays mentioned in the text (A–D), and cell cycle progression tested by PI-FACS (E and F), with cyclin D1 and cdc2 expression tested and quantified (G). For each assay, n=5. *p<0.05 vs. “Pare” cells. Experiments in this figure were repeated four times, and similar results were obtained.