Research Paper Volume 11, Issue 23 pp 11136—11147

Ninjurin2 overexpression promotes glioma cell growth

Figure 7. Ectopic Ninj2 overexpression promotes glioma cell progression in vitro. A172 cells were infected with the Ninj2-cDNA lentivirus (LV-Ninj2), followed by puromycin selection two stable cell lines (“SL1/SL2”) were established (“OE-Ninj2” cells). Control cells were infected with empty vector lentivirus (“Vec”); Expression of listed genes were tested by qPCR and Western blotting (AD); Cell viability (MTT OD, E) and soft agar colony formation (F) were tested; Cell proliferation was tested by cell counting assay (G) and EdU staining assay (H), with cell migration tested by the Transwell assay (I). Expression of listed proteins was quantified and normalized to the loading control (C and D). For each assay, n=5. *p<0.05 vs. “Vec” cells. Experiments in this figure were repeated three times, and similar results were obtained.