Figure 2. Overexpression of ciRs-6 suppresses bladder cancer growth in vitro and in vivo. (A) The levels of ciRs-6 and SLC41A2 in ciRs-6 overexpressed bladder cancer cells were determined by qPCR; (B, C) CCK8 assays were performed to evaluate cell viability; (D, E) clone formation assays were used to evaluate the ability to form clones; (F, G) cells in the S phase were assessed by cell cycle analysis; (H–J) mouse subcutaneous tumor model was used to detect the suppressive role of ciRs-6 in tumor growth in vivo. Each group contained 5 mice. The results are displayed as the mean±SEM, *p<0.05, **p<0.01.